Required Practical - Decay

Biology: AQA GCSE Higher, topic 7.2.15

Practical - Temperature & Milk Decay

Investigate the effect of temperature on the rate of decay of fresh milk by measuring pH change.

Outline

Outline

  • The effect of temperature on the rate of decay can be observed using milk.
  • As milk takes a long time to decay, lipase and sodium carbonate are added to speed up the decay.
  • As milk decays, its pH reduces. This can be observed with an indicator called Cresol red.
Method

Method

  • Use a water bath to heat all of the components to a given temperature.
  • Mix the milk, sodium carbonate and Cresol red. The resulting solution should be purple.
  • Add lipase to the solution and start timing.
  • When the solution turns yellow, stop timing.
  • Repeat using different temperatures.
Independent and dependent variable

Independent and dependent variable

  • The independent variable is the temperature.
  • The dependent variable is the time taken for the solution to turn yellow.

Test yourself on Required Practical - Decay

  • What is the independent variable in an experiment investigating the effect of temperature on the rate of decay of fresh milk by measuring pH change?

  • Which substances can be added to speed up the decay of milk?

  • What is the name of the indicator used to detect the changing pH of milk?

  • What piece of equipment is used to heat the milk mixture to a set temperature in this experiment?

  • Why does the solution change colour from purple to yellow during this experiment?

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