Rate of Enzyme Action

Biology: Cambridge International GCSE Supplement, topic 5.1.2

Factors Affecting the Rate of Enzyme Action

The factors that affect the rate (speed) of enzyme action are:

Temperature

Temperature

  • Increasing the temperature of a working enzyme initially increases the reacting activity. Molecules have more kinetic energy, so there are more frequent collisions (meetings) between substrates and enzymes.
  • Enzymes have an optimum temperature. Once this temperature is reached, the activity decreases.
  • Past a certain temperature, the active site changes shape, and the enzyme is denatured (loses its catalytic activity).
pH (measure of acidity)

pH (measure of acidity)

  • Enzymes have an optimum pH (measure of acidity).
  • If the pH changes away from the optimum pH, then the enzyme activity decreases.
  • If the pH is too low or too high, then the enzyme is denatured and will not function.

Test yourself on Rate of Enzyme Action

  • What happens to enzymes when pH is very high?

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