Investigating Heart Rates

Test yourself

Investigating Heart Rates

The effect of different substances on heart rates can be investigated using Daphnia crustaceans.

Daphnia

Daphnia are small, transparent crustaceans that can be used to investigate the effect of different chemicals on heart rates.
- Because they are transparent, it is easy to count the heart rate of Daphnia.

Experiment

To conduct this experiment, six Daphnia are placed in beakers of water and cooled.
- Cooling helps lower the heart rate, making it easier to count.
The heart rate of all six Daphnia are measured prior to being placed into the beakers.
- Three Daphnia are then placed in beakers with equal volumes of pond water. This is the control group.
- Three Daphnia are placed in beakers with equal volumes of pond water + 0.5% caffeine. This is the experimental group.

Experiment - 2

All six Daphnia are then left for 30 minutes.
After 30 minutes the heart rate of each Daphnia is counted for 30 seconds.
All conditions apart from the addition of caffeine should remain constant throughout the experiment.
- These conditions include temperature, glucose concentration, the volume of pond water, and size of the beaker.

Results

The results are shown in the table. Some important points to note are:
- The Daphnia in just pond water are the control group. This is to ensure any change in heart rate is due to the addition of caffeine.
- Three Daphnia are used in each group as it allows the mean heart rate to be calculated.
- This allows the detection of any anomalous results.
  - An anomalous result does not fit the pattern of the other results. This may be due to an experimental error.

Results

Interpreting results

As you can see from the results, adding caffeine increases the heart rate of Daphnia.
This is because caffeine is positively chronotropic.
- Positive chronotropes increase the heart rate.
Caffeine does this by inhibiting specific receptors in the heart. Stimulation of these receptors normally decreases heart rates.
- So, when caffeine inhibits these receptors, heart rate increases.

1Principles of Science I

1.1Structure & Bonding

1.1.1Atomic Model

1.1.2Electron Shells, Sub-Shells & Orbitals

1.1.3Ionic Bonding

1.1.4Representing Ionic Bonds

1.1.5Covalent Bonding

1.1.6Representing Covalent Bonds

1.1.7Metallic Bonding

1.1.8Intermolecular Forces

1.1.9Intermolecular Forces 2

1.1.10End of Topic Test - Bonding

1.1.11Relative Masses

1.1.12The Mole

1.1.13Molar Calculations

1.1.14Molar Calculations 2

1.1.15Empirical & Molecular Formulae

1.1.16Balanced Equations

1.1.17Percentage Yield

1.1.18End of Topic Test - Amount of Substance

1.2Properties of Substances

1.2.1The Periodic Table

1.2.2Ionisation Energy

1.2.3Factors Affecting Ionisation Energies

1.2.4Trends of Ionisation

1.2.5Trends in the Periodic Table

1.2.6Polarity

1.2.7Metals & Non-Metals

1.2.8Alkali Metals

1.2.9Alkaline Earth Metals

1.2.10Reactivity of Alkaline Earth Metals

1.2.11Redox

1.2.12Transition Metals

1.2.13Redox Reactions of Transition Metals

1.3Cell Structure & Function

1.3.1Introduction to Cells

1.3.2Prokaryotes

1.3.3Organelles of Eukaryotic Cells

1.3.4Organelles of Eukaryotic Cells 2

1.3.5Organelles in Plant Cells

1.3.6Microscopy

1.3.7End of Topic Test - Cell Structure

1.4Cell Specialisation

1.4.1Cell Specialisation

1.4.2Blood Cells

1.4.3Plant Cells

1.4.4Gametes

1.5Tissue Structure & Function

1.5.1Human Gas Exchange

1.5.2Blood Vessels

1.5.3Atherosclerosis

1.5.4Skeletal Muscle

1.5.5Slow & Fast Twitch Fibres

1.5.6Neurones

1.5.7Speed of Transmission

1.5.8Action Potentials

1.5.9End of Topic Test - Neurones & Action Potentials

1.5.10Synapses

1.5.11Types of Synapse

1.5.12Medical Application

1.5.13End of Topic Test - Synapses

1.5.14Chemical Brain Imbalances

1.5.15Effect of Drugs on the Brain

1.6Working with Waves

1.6.1Progressive Waves

1.6.2Wave Speed & Phase Difference

1.6.3Longitudinal & Transverse Waves

1.6.4Stationary Waves

1.6.5Stationary Waves 2

1.6.6Applications of Stationary Waves

1.6.7Interference

1.6.8Diffraction

1.6.9Diffraction Gratings

1.6.10Application of Diffraction Gratings

1.7Waves in Communication

1.7.1Refraction at a Plane Surface

1.7.2Internal Reflection & Fibre Optics

1.7.3Applications of Total Internal Reflection

1.7.4Properties

1.7.5Gamma, X-Ray & UV Light

1.7.6Visible Light

1.7.7Infrared, Microwave & Radio Waves

1.7.8Intensity

1.7.9Digital & Analogue Signals

1.7.10Communication Channels

2Practical Scientific Procedures and Techniques

2.1Titration

2.1.1pH Curves & Titrations

2.1.2pH Curves & Titrations 2

2.1.3Buffer Solutions

2.2Colorimetry

2.2.1Measuring Rates

2.2.2Beer-Lambert Law

2.3Chromatography

2.3.1Overview of Chromatography

2.3.2Thin-Layer Chromatography

2.3.3Column Chromatography

2.3.4Gas Chromatography

3Science Investigation Skills

3.1Scientific Processes

3.1.1Aims, Hypotheses & Sampling

3.1.2Pilot Studies & Design

3.1.3Ethics

3.1.4Variables & Control

3.1.5Demand Characteristics & Investigator Effects

3.1.6Features of Science

3.1.7Scientific Report

3.1.8Scientific Report 2

3.2Data Handling & Analysis

3.2.1Types of Data

3.2.2Descriptive Statistics

3.2.3Presentation & Display of Data

3.3Enzymes in Action

3.3.1Overview of Protein Structure

3.3.2Enzymes

3.3.3Factors Affecting Enzyme Activity

3.3.4Enzyme-Controlled Reactions

3.4Diffusion

3.4.1Diffusion

3.5Plants & Their Environment

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3.6Energy Content in Fuels

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3.7.3Resistance

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3.7.6Domestic Uses of Electricity

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4Principles of Science II

4.1Extracting Elements

4.1.1Atom Economy

4.2Relating Properties to use of Substances

4.2.1Group 2 Chemistry

4.2.2End of Topic Test - Group 2

4.2.3Electrolysis

4.2.4Electrolysis Examples

4.2.5Extracting Metals

4.2.6Transition Metals

4.2.7Variable Oxidation States

4.2.8Heterogeneous Catalysts

4.2.9Homogeneous Catalysts

4.3Organic Chemistry

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4.3.2Functional Groups

4.3.3Isomerism

4.3.4Hydrocarbons

4.3.5Structure of Alkanes

4.3.6Boiling Points of Alkanes

4.3.7Alkenes

4.3.8Curly Arrows & Mechanisms

4.3.9Cracking

4.3.10Combustion

4.3.11Free Radical Substitution of Alkanes

4.3.12Electrophilic Addition of Alkenes

4.4Energy Changes in Industry

4.4.1The Kelvin Scale

4.4.2Enthalpy Changes

4.4.3Calorimetry

4.4.4Bond Enthalpies

4.4.5Hess' Law

4.5The Circulatory System

4.5.1The Circulatory System

4.5.2Blood Vessels

4.5.3Blood Transfusion & the ABO Rhesus System

4.5.4The Heart

4.5.5The Cardiac Cycle

4.5.6Cardiac Output

4.5.7Coordination of Heart Action

4.5.8Heart Dissection

4.5.9Controlling Heart Rate

4.5.10Electrocardiograms

4.5.11Cardiovascular Disease

4.5.12Investigating Heart Rates

4.6Ventilation & Gas Exchange

4.6.1Human Gas Exchange

4.6.2Ventilation

4.6.3Measuring Gas Exchange

4.6.4Controlling Ventilation Rate

4.6.5Lung Disease

4.6.6Lung Disease Data

4.7Urinary System

4.7.1Kidneys & Control of Water Balance

4.7.2Kidneys & Osmoregulation

4.7.3Kidneys & Blood Pressure

4.7.4Urine

4.7.5Kidney Failure & Urinalysis

4.7.6Dialysis

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4.8Cell Transport

4.8.1Cell Membrane Structure

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4.8.3Osmosis

4.8.4Active Transport

4.9Thermal Physics

4.9.1Power & Efficiency

4.9.2Work & Energy

4.9.3Conservation of Energy

4.9.4Pressure

4.9.5First Law of Thermodynamics

4.9.6Second Law of Thermodynamics

4.9.7Heat Engines, Heat Pumps & Refrigerators

4.9.8Non-Flow Processes

4.9.9p-V Diagrams

4.9.10Ideal Gases

4.9.11Ideal Gases 2

4.9.12Thermal Energy Transfer

4.9.13Thermal Energy Transfer Experiments

4.10Materials

4.10.1Density

4.10.2Stress & Strain

4.10.3Properties of Materials

4.10.4Elasticity

4.10.5Plasticity

4.10.6Energy in Materials

4.10.7Young Modulus

4.11Fluids

4.11.1Fluid Flow

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4.11.4Continuity Equation

4.11.5Bernoulli's Equation

4.11.6Non-Newtonian Fluids

5Contemporary Issues in Science

5.1Contemporary Issues in Science

5.1.1Sustainability

5.1.2Genetic Engineering

5.1.3Uses of Genetic Modification

5.1.4Stem Cell Therapy

5.1.5Cloning

5.1.6Cloning 2

5.1.7Nanotechnology

5.1.8Ecosystems

5.2Analysing Scientific Information

5.2.1Validity

5.2.2Peer Review

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