1.3.6

Microscopy

Test yourself

Magnification and Resolution

Two parameters that are important in microscopy are magnification and resolution.

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Magnification

  • Magnification is the process of enlarging an object in appearance.
  • The image size is how big the object appears to be in a picture or drawing, which will be in milimeters (mm).
  • The actual size is often given in micrometers (µm) - units must be converted so that they are the same.
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Calculating magnification

  • The equation for magnification is:
    • Magnification = size of image ÷ size of real object
  • E.g. A mitochondrion is 20 µm long. An image of the mitochondrion is measured as 20 mm long. What is the magnification?
    • Magnification = 20,000 µm ÷ 20 µm = 1,000x
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Resolution

  • Resolution is the ability of a microscope to distinguish two adjacent structures as separate.
  • The higher the resolution, the better the clarity and detail of the image.

Jump to other topics

1Principles of Science I

1.1Structure & Bonding

1.2Properties of Substances

1.3Cell Structure & Function

1.4Cell Specialisation

1.5Tissue Structure & Function

1.6Working with Waves

1.7Waves in Communication

2Practical Scientific Procedures and Techniques

3Science Investigation Skills

4Principles of Science II

4.1Extracting Elements

4.2Relating Properties to use of Substances

4.3Organic Chemistry

4.4Energy Changes in Industry

4.5The Circulatory System

4.6Ventilation & Gas Exchange

4.7Urinary System

4.8Cell Transport

4.9Thermal Physics

4.10Materials

4.11Fluids

5Contemporary Issues in Science

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