3.9.2

High-Performance Liquid Chromatography

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High-Performance Liquid Chromatography

High-performance liquid chromatography (HPLC) is a type of column chromatography.

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HPLC

  • HPLC takes place in a column.
    • The stationary phase is silica.
    • The mobile phase can be chosen to best suit the compounds being separated.
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How does it work?

  • HPLC pushes the compounds down the column, and they are separated.
  • They elute (come out) at different times depending on their Rf values.
    • By collecting the solution in different flasks you can separate the compounds.
    • The time taken for a compound to elute is known as its retention time.

Jump to other topics

1Physical Chemistry

1.1Atoms, Molecules & Stoichiometry

1.2Atomic Structure

1.3Chemical Bonding

1.4States of Matter

1.5Chemical Energetics

1.6Electrochemistry

1.7Equilibria

1.8Partition Coefficient

1.9Reaction Kinetics

2Inorganic Chemistry

3Organic Chemistry & Analysis

3.1Introduction to Organic Chemistry

3.2Hydrocarbons

3.3Halogen Derivatives

3.4Hydroxy Compounds

3.5Carbonyl Compounds

3.6Carboxylic Acids & Derivatives

3.7Nitrogen Compounds

3.8Polymerisation

3.9Analytical Techniques

3.10Organic Synthesis

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