2.2.6

Investigating Effect of pH on Amylase

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Investigating the Effect of pH on Amylase

Amylase breaks down starch, which can be detected using iodine solution.

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Measuring reaction time

  • Add amylase to starch solution.
  • Every 30 seconds, take a drop of the mixture and add a drop of iodine solution.
  • Record the total time taken for the iodine solution to stop turning blue when added (which indicates that all the starch has been broken down).
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Finding amylase's optimal pH

  • Repeat this experiment using starch solutions with different pHs.
  • The shorter the time taken for the iodine to stop changing colour, the faster the amylase has broken down the starch and the closer this pH is to amylase's optimal pH.

Jump to other topics

1The Nature & Variety of Living Organisms

2Structure & Functions in Living Organisms

3Organism Functions

3.1Respiration

3.2Gas Exchange

3.3Transport

3.4Excretion

3.5Coordination & Response

4Reproduction & Inheritance

5Ecology

6Biological Resources

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