2.1.8

Chromatography

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Paper Chromatography

In paper chromatography, mixtures of soluble substances are separated. A solvent (mobile phase) is run through the mixture on paper (containing the stationary phase). The substances will then move up the paper at different rates. The most soluble substance will move the furthest up the paper.

Dip the chromatography paper

Dip the chromatography paper

Dip the bottom of the chromatography paper into the solvent.

Solvent movement

Solvent movement

The solvent travels up the chromatography paper.
As the solvent moves, it picks up the substances being tested and carries them up the chromatography paper.

Distance travelled

Distance travelled

The components that travel furthest are highly soluble (dissolve easily) in the solvent. They are also minimally attracted to the chromatography paper.

1

Biology

1.1

Practicals

1.1.1Microscope & Bacteria 1.1.2Bacteria 1.1.3Osmosis Tests 1.1.4Osmosis & Food Tests 1.1.5Enzymes 1.1.6Enzymes 2 1.1.7Photosynthesis & Plant Responses 1.1.8Photosynthesis Experiments 1.1.9Reaction Time 1.1.10Quadrats 1.1.11Decay

2

Chemistry

2.1

Practicals

2.1.1Salts 2.1.2Neutralisation 2.1.3Titration 2.1.4Electrolysis 2.1.5Electrolysis 2 2.1.6Rate of Reaction 2.1.7Rate of Reaction 2 2.1.8Chromatography 2.1.9Identifying Gases 2.1.10Identifying Gases 2 2.1.11Identifying Ions 2.1.12Carbonates, Halides & Sulfates 2.1.13Flame Emission Spectroscopy 2.1.14Water Purification 2.1.15Water Purification 2

3

Physics

3.1

Practicals

3.1.1Specific Heat Capacity 3.1.2Thermal Capacity & Insulation 3.1.3Latent Heat 3.1.4Resistance & Current 3.1.5Resistance & Current 2 3.1.6Density 3.1.7Density 2 3.1.8Force & Extension - Springs 3.1.9Waves - Ripple Tank 3.1.10Light 3.1.11Light Reflection & Refraction 3.1.12Radiation 3.1.13Radiation 2

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1

Biology

1.1

Practicals

1.1.1Microscope & Bacteria 1.1.2Bacteria 1.1.3Osmosis Tests 1.1.4Osmosis & Food Tests 1.1.5Enzymes 1.1.6Enzymes 2 1.1.7Photosynthesis & Plant Responses 1.1.8Photosynthesis Experiments 1.1.9Reaction Time 1.1.10Quadrats 1.1.11Decay

2

Chemistry

2.1

Practicals

2.1.1Salts 2.1.2Neutralisation 2.1.3Titration 2.1.4Electrolysis 2.1.5Electrolysis 2 2.1.6Rate of Reaction 2.1.7Rate of Reaction 2 2.1.8Chromatography 2.1.9Identifying Gases 2.1.10Identifying Gases 2 2.1.11Identifying Ions 2.1.12Carbonates, Halides & Sulfates 2.1.13Flame Emission Spectroscopy 2.1.14Water Purification 2.1.15Water Purification 2

3

Physics

3.1

Practicals

3.1.1Specific Heat Capacity 3.1.2Thermal Capacity & Insulation 3.1.3Latent Heat 3.1.4Resistance & Current 3.1.5Resistance & Current 2 3.1.6Density 3.1.7Density 2 3.1.8Force & Extension - Springs 3.1.9Waves - Ripple Tank 3.1.10Light 3.1.11Light Reflection & Refraction 3.1.12Radiation 3.1.13Radiation 2

Practice questions on Chromatography

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1
In paper chromatography, the components that travel furthest are...True / false
2
What is the mobile phase in paper chromatography?Multiple choice
3
What can we say if the Rf values of a reference and a spot match in all solvents?Multiple choice
4
Which of the following describe a reference substance?True / false
5
What does the process of chromatography produce that can be analysed?Multiple choice

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Rate of Reaction 2

Identifying Gases