High-Performance Liquid Chromatography
Chemistry: Cambridge International A Level, topic 3.9.2
High-Performance Liquid Chromatography
High-performance liquid chromatography (HPLC) is a type of column chromatography.
HPLC
- HPLC takes place in a column.
- The stationary phase is silica.
- The mobile phase can be chosen to best suit the compounds being separated.
How does it work?
- HPLC pushes the compounds down the column, and they are separated.
- They elute (come out) at different times depending on their Rf values.
- By collecting the solution in different flasks you can separate the compounds.
- The time taken for a compound to elute is known as its retention time.
Test yourself on High-Performance Liquid Chromatography
High-Performance Liquid Chromatography (HPLC)
Which of the following statements about HPLC are true?