Measuring Number & Distribution of Species
Applied Science: BTEC Level 3 National Extended Diploma, topic 3.5.2
Quadrats
The population size of immobile (e.g. plants) or slow-moving organisms (e.g. limpets) can be estimated using random samples of quadrats.

Quadrats & point frames
- Quadrats are normally 1m2 square frame divided into 100 squares.
- Each square represents 1% of the total area of the quadrat.
- If an individual covers more than half of a square, it is counted as representing 1% of the quadrat.
- Quadrats are used to count the population size within a large area.
- Some quadrants can have a point frame attached.
- A point frame contains ten pins. When placed on a quadrat, you note down the plant species each pin touches.
- Point frames can be used to measure plant cover & distribution.

Sampling
- If you wanted to investigate the size of a population, it would take hours to count every individual.
- Taking samples (a small section of the total area) allows the population size to be estimated.
- It is important that the samples accurately represent the total population size.

Random sampling
- Random sampling can be applied by dividing the area of interest into a grid and labelling the grid with coordinates.
- A random number generator provides a set of random coordinates.
- The quadrats can be placed using the coordinates.

Transects
- Quadrats could also be placed at regular intervals along a transect.
- A transect is a line across a habitat, usually placed using a tape measure.
- Transects are used when there is a change, or gradient, in abiotic conditions across the habitat.
- Transects are used when looking for changes in the abundance or distribution of a species across the environment (e.g. how abundance changes from open field to forest).

Line transect
- A line transect records the species that makes contact with the tape measure at regular intervals along the transect.

Belt transect
- A belt transect uses quadrats.
- Quadrats are placed at regular intervals along the transect.
- An abundance of different species in the quadrat can be measured by:
- Percentage cover – estimate how much of the quadrat is covered by a particular species
- Frequency – count how many individuals of a particular species are present in the quadrat.
Test yourself on Measuring Number & Distribution of Species
Which of the following statements about quadrats are true?
In what sort of habitat are transects used?