DNA Technology

Biology: IB Diploma Higher Level, topic 13.3.3

Evolutionary Relationships

Advances in technology have led to the development of new techniques to help establish evolutionary relationships between species. These techniques include:

Genome sequencing

Genome sequencing

  • The base sequences of organisms can be sequenced and compared to other organisms.
  • The more similar the base sequences are between organisms, the more closely related the two organisms are.
  • Genome sequencing has helped clarify evolutionary relationships (e.g. the relationship between skunks and weasels).
Amino acid sequencing

Amino acid sequencing

  • The amino acids that make-up proteins in an organism can be sequenced and compared to other organisms.
  • The more similar the amino acid sequences are between organisms, the more closely related they are.
  • E.g. Cytochrome C is an electron carrier protein used in respiration (it is in many organisms). Comparing the amino acid sequence of cytochrome C between organisms clarifies evolutionary relationships.
Antibody-antigen interactions

Antibody-antigen interactions

  • Species produce specific antibodies that recognise foreign proteins.
  • An organism's antibodies can be isolated and mixed with another organism's proteins to see how many antigen-antibody complexes are formed.
  • The more complexes that form, the more closely related the two organisms are.

Test yourself on DNA Technology

  • Which of the following statements about DNA technology are true?

  • Which of these is NOT a DNA technique that can be used to clarify evolutionary relationships?

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