Diagnostic Misconceptions - Villain
English Lit: AQA GCSE Macbeth, topic 3.2.4

Lady Macbeth is manipulative and ruthless, but she’s also vulnerable. She concedes that she doesn’t have it in her to kill Duncan herself because he resembles her ‘father as he slept’ and, later on, she becomes increasingly concerned by Macbeth’s tyranny. And then there’s Act 5 Scene 1: ‘Out, damned spot!’
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How does Macbeth's tyranny affect Lady Macbeth throughout the play?