Source: https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-8-chromatography [Revision notes](https://senecalearning.com/en-gb/revision-notes.md) › [GCSE](https://senecalearning.com/en-gb/revision-notes/gcse.md) › [Science Practicals](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals.md) › [National Curriculum](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum.md) › Chromatography # Chromatography Science Practicals: GCSE, topic 2.1.8 ## Paper Chromatography In paper chromatography, mixtures of soluble substances are separated. A solvent (mobile phase) is run through the mixture on paper (containing the stationary phase). The substances will then move up the paper at different rates. The most soluble substance will move the furthest up the paper. ### Dip the chromatography paper ![Dip the chromatography paper](https://image-v2.cdn.app.senecalearning.com/courseImages/chemistry/8.1.1%20Purity%2C%20formulations%20and%20chromatography/dips-58738_640-min.jpg) - Dip the bottom of the chromatography paper into the solvent. ### Solvent movement ![Solvent movement](https://image-v2.cdn.app.senecalearning.com/courseImages/chemistry/8.1.1%20Purity%2C%20formulations%20and%20chromatography/png-2757379_640.png) - The solvent travels up the chromatography paper. - As the solvent moves, it picks up the substances being tested and carries them up the chromatography paper. ### Distance travelled ![Distance travelled](https://image-v2.cdn.app.senecalearning.com/courseImages/chemistry/8.1.1%20Purity%2C%20formulations%20and%20chromatography/aircraft-2795557_640.jpg) - The components that travel furthest are highly soluble (dissolve easily) in the solvent. They are also minimally attracted to the chromatography paper. ## Test yourself on Chromatography - In paper chromatography, the components that travel furthest are... - A student uses a gas as one of the phases in a chromatography experiment. Which phase must this gas be? - What can we say if the Rf values of a reference and a spot match in all solvents? - Which of the following describe a reference substance? - What does the process of chromatography produce that can be analysed? [Practise this topic for free](https://app.senecalearning.com/classroom/course/35d9e7b0-1dfb-11e8-b5b7-7546c70e1a36/section/598b0ba0-1e03-11e8-b5b7-7546c70e1a36/session) - Previous topic: [2.1.7 Rate of Reaction 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-7-rate-of-reaction-2.md) - Next topic: [2.1.9 Identifying Gases](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-9-identifying-gases.md) ## Science Practicals: GCSE contents ### 1 Biology - 1.1 Practicals - [1.1.1 Microscope & Bacteria](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-1-microscope-and-bacteria.md) - [1.1.2 Bacteria](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-2-bacteria.md) - [1.1.3 Osmosis Tests](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-3-osmosis-tests.md) - [1.1.4 Osmosis & Food Tests](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-4-osmosis-and-food-tests.md) - [1.1.5 Enzymes](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-5-enzymes.md) - [1.1.6 Enzymes 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-6-enzymes-2.md) - [1.1.7 Photosynthesis & Plant Responses](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-7-photosynthesis-and-plant-responses.md) - [1.1.8 Photosynthesis Experiments](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-8-photosynthesis-experiments.md) - [1.1.9 Reaction Time](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-9-reaction-time.md) - [1.1.10 Quadrats](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-10-quadrats.md) - [1.1.11 Decay](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-11-decay.md) ### 2 Chemistry - 2.1 Practicals - [2.1.1 Salts](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-1-salts.md) - [2.1.2 Neutralisation](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-2-neutralisation.md) - [2.1.3 Titration](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-3-titration.md) - [2.1.4 Electrolysis](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-4-electrolysis.md) - [2.1.5 Electrolysis 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-5-electrolysis-2.md) - [2.1.6 Rate of Reaction](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-6-rate-of-reaction.md) - [2.1.7 Rate of Reaction 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-7-rate-of-reaction-2.md) - [2.1.8 Chromatography](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-8-chromatography.md) - [2.1.9 Identifying Gases](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-9-identifying-gases.md) - [2.1.10 Identifying Gases 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-10-identifying-gases-2.md) - [2.1.11 Identifying Ions](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-11-identifying-ions.md) - [2.1.12 Carbonates, Halides & Sulfates](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-12-carbonates-halides-and-sulfates.md) - [2.1.13 Flame Emission Spectroscopy](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-13-flame-emission-spectroscopy.md) - [2.1.14 Water Purification](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-14-water-purification.md) - [2.1.15 Water Purification 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-15-water-purification-2.md) ### 3 Physics - 3.1 Practicals - [3.1.1 Specific Heat Capacity](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-1-specific-heat-capacity.md) - [3.1.2 Thermal Capacity & Insulation](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-2-thermal-capacity-and-insulation.md) - [3.1.3 Latent Heat](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-3-latent-heat.md) - [3.1.4 Resistance & Current](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-4-resistance-and-current.md) - [3.1.5 Resistance & Current 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-5-resistance-and-current-2.md) - [3.1.6 Density](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-6-density.md) - [3.1.7 Density 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-7-density-2.md) - [3.1.8 Force & Extension - Springs](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-8-force-and-extension-springs.md) - [3.1.9 Waves - Ripple Tank](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-9-waves-ripple-tank.md) - [3.1.10 Light](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-10-light.md) - [3.1.11 Light Reflection & Refraction](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-11-light-reflection-and-refraction.md) - [3.1.12 Radiation](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-12-radiation.md) - [3.1.13 Radiation 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-13-radiation-2.md)