Source: https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-2-bacteria [Revision notes](https://senecalearning.com/en-gb/revision-notes.md) › [GCSE](https://senecalearning.com/en-gb/revision-notes/gcse.md) › [Science Practicals](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals.md) › [National Curriculum](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum.md) › Bacteria # Bacteria Science Practicals: GCSE, topic 1.1.2 ## Growing Microorganisms Cultures of microorganisms (microorganisms grown in a lab) can be used to investigate the effects of antibiotics and disinfectants, which both kill bacteria. Microorganisms can be grown in two mediums (materials) that contain everything that bacteria need to survive. ### Agar gel plate ![Agar gel plate](https://image-v2.cdn.app.senecalearning.com/courseImages/biology/1.1.4/agar-plate-min.jpg) - Microorganisms can be grown as colonies on an agar gel plate. ### Nutrient broth ![Nutrient broth](https://image-v2.cdn.app.senecalearning.com/courseImages/biology/1.1.4/nutrient-borth-min.jpg) - Microorganisms can be grown within a solution of a nutrient broth. - This contains carbohydrates as an energy source, minerals and sometimes other chemicals. ## Calculating Area The equation for calculating the area of a circle is: Area = π x radius2. ### Clear-zones ![Clear-zones](https://image-v2.cdn.app.senecalearning.com/courseImages/biology/OCR%2021%20Century/2%20Health/clear_zone_bacteria.jpg) - Samples of certain antiseptics or antibiotics can be added to bacterial cultures. The area free of bacteria around the sample can then be calculated. - This technique is useful for testing how well different antibiotics work. It's also useful for identifying bacteria or other microorganisms based on the antimicrobials that affect their growth. ### Bacterial cultures ![Bacterial cultures](https://image-v2.cdn.app.senecalearning.com/courseImages/biology/OCR%2021%20Century/2%20Health/culturing_microorganisms.jpg) - The size of bacterial cultures can be measured by the area of the colony. - This is useful for determining how different conditions affect bacterial growth rates. ## Test yourself on Bacteria - Bacteria can be cultured in several ways. Which of the following is NOT a method? - What equation would be used to calculate the areas of bacteria killed by the antiseptic/antibiotic, and what would this indicate? - How do bacteria multiply? - Which of these clear-zone areas (the area free of bacteria) was made by the best antibiotic? [Practise this topic for free](https://app.senecalearning.com/classroom/course/35d9e7b0-1dfb-11e8-b5b7-7546c70e1a36/section/d8ed47a2-b4b2-45d9-915c-b324dc98178c/session) - Previous topic: [1.1.1 Microscope & Bacteria](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-1-microscope-and-bacteria.md) - Next topic: [1.1.3 Osmosis Tests](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-3-osmosis-tests.md) ## Science Practicals: GCSE contents ### 1 Biology - 1.1 Practicals - [1.1.1 Microscope & Bacteria](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-1-microscope-and-bacteria.md) - [1.1.2 Bacteria](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-2-bacteria.md) - [1.1.3 Osmosis Tests](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-3-osmosis-tests.md) - [1.1.4 Osmosis & Food Tests](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-4-osmosis-and-food-tests.md) - [1.1.5 Enzymes](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-5-enzymes.md) - [1.1.6 Enzymes 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-6-enzymes-2.md) - [1.1.7 Photosynthesis & Plant Responses](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-7-photosynthesis-and-plant-responses.md) - [1.1.8 Photosynthesis Experiments](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-8-photosynthesis-experiments.md) - [1.1.9 Reaction Time](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-9-reaction-time.md) - [1.1.10 Quadrats](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-10-quadrats.md) - [1.1.11 Decay](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/1-1-11-decay.md) ### 2 Chemistry - 2.1 Practicals - [2.1.1 Salts](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-1-salts.md) - [2.1.2 Neutralisation](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-2-neutralisation.md) - [2.1.3 Titration](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-3-titration.md) - [2.1.4 Electrolysis](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-4-electrolysis.md) - [2.1.5 Electrolysis 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-5-electrolysis-2.md) - [2.1.6 Rate of Reaction](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-6-rate-of-reaction.md) - [2.1.7 Rate of Reaction 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-7-rate-of-reaction-2.md) - [2.1.8 Chromatography](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-8-chromatography.md) - [2.1.9 Identifying Gases](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-9-identifying-gases.md) - [2.1.10 Identifying Gases 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-10-identifying-gases-2.md) - [2.1.11 Identifying Ions](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-11-identifying-ions.md) - [2.1.12 Carbonates, Halides & Sulfates](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-12-carbonates-halides-and-sulfates.md) - [2.1.13 Flame Emission Spectroscopy](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-13-flame-emission-spectroscopy.md) - [2.1.14 Water Purification](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-14-water-purification.md) - [2.1.15 Water Purification 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/2-1-15-water-purification-2.md) ### 3 Physics - 3.1 Practicals - [3.1.1 Specific Heat Capacity](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-1-specific-heat-capacity.md) - [3.1.2 Thermal Capacity & Insulation](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-2-thermal-capacity-and-insulation.md) - [3.1.3 Latent Heat](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-3-latent-heat.md) - [3.1.4 Resistance & Current](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-4-resistance-and-current.md) - [3.1.5 Resistance & Current 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-5-resistance-and-current-2.md) - [3.1.6 Density](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-6-density.md) - [3.1.7 Density 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-7-density-2.md) - [3.1.8 Force & Extension - Springs](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-8-force-and-extension-springs.md) - [3.1.9 Waves - Ripple Tank](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-9-waves-ripple-tank.md) - [3.1.10 Light](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-10-light.md) - [3.1.11 Light Reflection & Refraction](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-11-light-reflection-and-refraction.md) - [3.1.12 Radiation](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-12-radiation.md) - [3.1.13 Radiation 2](https://senecalearning.com/en-gb/revision-notes/gcse/science-practicals/national-curriculum/3-1-13-radiation-2.md)